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    目錄:愛必信(上海)生物科技有限公司>>抗體>>其它一抗>> abs119600Rabbit anti-β-Actin Polyclonal Antibody

    Rabbit anti-β-Actin Polyclonal Antibody

    • Rabbit anti-β-Actin Polyclonal Antibody
    參考價1500-2300
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    參考價:¥1500 ~ ¥2300

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    • absin 品牌
    • abs119600 型號
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    規格

    50uL 100uL

    屬性

    供貨周期:現貨 規格:50uL;100uL 貨號:abs119600 主要用途:WB: 1:5000-50000, ICC: 1:100

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    100uL2300元999uL可售

    更新時間:2025-10-28 15:36:07瀏覽次數:2287評價

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    供貨周期 現貨 規格 50uL;100uL
    貨號 abs119600 主要用途 WB: 1:5000-50000, ICC: 1:100
    Rabbit anti-β-Actin Polyclonal Antibody,宿主Rabbit,特異性beta-Actin (Loading Control),應用WB: 1:5000-50000, ICC: 1:100, FCM: 1ug/T, ELISA: 1:5000-20000
    概述
    別名
    β Actin抗體;Beta Actin; beta-Actin; ACTB; Actin cytoplasmic 1; Actin; beta; Beta actin; beta cytoskeletal actin; A X actin like protein; alpha sarcomeric Actin; Actx; Beta cytoskeletal actin; Melanoma X actin; PS1TP5BP1; ACTB_HUMAN,betaActin
    宿主
    Rabbit
    特異性
    beta-Actin (Loading Control)
    反應種屬
    Human, Mouse, Rat, Hamster
    預測反應種屬
    Chicken, Dog, Pig, Rabbit, Sheep, Bee, Fish, Guinea Pig, Cat
    應用
    WB: 1:5000-50000, IHC-F: 1:200-1000, IHC-P: 1:200-1000, IF: 1:200-1000, ICC/IF: 1:100-500, FCM: 1ug/Test
    分子量
    Predicted molecular weight: 42kDa
    免疫原
    Synthetic MAP peptide derived from human beta-Actin(1-200/375)
    性能
    形式
    Liquid
    濃度
    1mg/mL
    純化方法
    affinity purified by Protein A
    類型
    Polyclonal Antibody
    克隆號
    同種型
    IgG
    儲存/保存方法
    Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    存儲溶液
    0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    標記
    Unlabeled
    修飾
    Unmodified
    靶標
    背景說明
    Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence. Antibodies against plant Actin are useful as loading controls for Western Blotting.
    組織特異性
    Ubiquitously expressed in all eukaryotic cells.
    翻譯后修飾
    ISGylated.
    Oxidation of Met-44 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced.
    細胞定位
    細胞漿
    UniProt
    P60709
    研究領域
    研究領域
    Signal TransductionCytoskeleton / ECMCytoskeletonMicrofilamentsActin etcActin
    Tags & Cell MarkersSubcellular MarkersCytoskeletonActin
    實驗結果圖
    Sample:Embryo Cerebrum (Mouse) Lysate at 40 ug Du145 (Human) Lysate at 40 ug SW480 (Human) Cell Lysate at 40 ug U87MG (Human) Lysate at 40 ug U251 (Human) Lysate at 40 ug A673 (Human) Lysate at 40 ug Lovo (Human) Lysate at 40 ug 293FT (Human) Lysate at 40 ug JEG-3 (Human) Lysate at 40 ug RSC96 (Rat) Cell Lysate at 40 ug MCF-7 (Human) Cell Lysate at 40 ug HepG2 (Human) Lysate at 40 ug A431 (Human) Lysate at 40 ug Primary: Anti-beta-Actin at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD
    MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (beta-Actin) polyclonal Antibody, Unconjugated 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
    Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (beta-Actin) polyclonal Antibody, Unconjugated 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG-FITC antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
    Sample: SH-SY5Y (Human) Lysate at 40 ug Primary: Anti-beta-Actin at 1/2000~1/20000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD
    Blank control: NIH/3T3. Primary Antibody (green line): Rabbit Anti-beta-Actin (Loading Control) antibody Dilution: 1ug/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1ug/T. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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