| I. Background | |||||||||||
| 鈣粘蛋白超家族是一組鈣依賴性膜表面糖蛋白分子，主要介導細胞粘附、胚胎發育， 并維持正常組織器官形態。CDH17 基因，即 cadherin-17，又稱 liver-intestine cadherin，是 鈣粘蛋白基因超家族的成員之一，編碼 Ca 2+依賴性的跨膜蛋白。CDH17 作為功能性 Ca 2+依 賴性細胞粘附分子發揮作用，但 是 CDH17 并不通過細胞內鈣連環蛋白與細胞支架的肌動 蛋白方式進行緊密連接作用，而通過直接與細胞支架連進行其細胞黏附作用。CDH17 結構的特異性決定了其發揮粘連作用的機制與經典的鈣粘連蛋白有所不同，故 CDH17 被 劃分為經典鈣黏蛋白的變異體。CDH17 在人十二指腸（伯納特腺除外）、回腸、結直腸以 及闌尾上皮細胞中表達，在胃體和胃竇等組織中不表達，但在腸上皮化生的胃黏膜偶有表 達。CDH17 在胃癌、結直腸癌、肝癌、胰腺癌和膽管癌等多種腫瘤組織中均有不同程度的 表達，CDH17 在多種腫瘤侵襲轉移中發揮重要作用。 | |||||||||||
| II. Introduction | |||||||||||
| Host Cell: | MC-38 | ||||||||||
| Expressed gene: | hCDH17 | ||||||||||
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) | ||||||||||
| Freeze Medium: | 90% FBS+10% DMSO | ||||||||||
| Culture Medium: | DMEM+10%FBS+2μg/ml Puromycin | ||||||||||
| Mycoplasma Testing: | Negative | ||||||||||
| Storage: | Liquid nitrogen | ||||||||||
| III. Description of Host Cell Line | |||||||||||
| Organism: | Mus musculus, mouse | ||||||||||
| Tissue: | Colon | ||||||||||
| Morphology: | Epitheloid cell | ||||||||||
| Growth Properties: | Adherent | ||||||||||
| IV. Representative Data | |||||||||||
Figure 1. Recombinant hCDH17/MC38 stably expressing CDH17. | |||||||||||
| V. In VIVO Data | |||||||||||
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1. Weight Change
Figure 2. hCDH17/MC38 Mean Body Weight ± SEM.
2. Tumor Size
Figure 3.hCDH17/MC38 Mean Tumor Volume ± SEM.
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NOTE：The above data was provided by Cobioer (cell data) and Truway Bio (in vivo data model).
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