單層應力加載單個細胞應力刺激系統

細胞被均勻地限制/壓縮在兩個亞微米分辨率的兩個平行表面之間。

不同的限制高度（例如1um – 300um）

允許長期細胞培養和細胞增殖，同時保持對封閉的控制

與高分辨率光學顯微鏡系統兼容

可以處理足夠多的細胞以進行完整的基因表達分析

可與生物功能化的微結構化底物和/或不同的基質（幾何形狀控制）結合使用

可以與凝膠結合（硬度控制）

兼容任何細胞培養底物（培養皿至96孔板）

 

產品：

 

應用：

Cell migration 2.5D, migration and interaction of non-adhesive cells, cell squeezing, imaging of flat cells (organelles aligned in 2D), super-resolution video-microscopy (organelles move less), contractility assay, etc

Confinement illustration
HeLa cells: not confined, 5 ?m, 3 ?m.

Explore examples of applications


> Cancer invasiveness assay: Quantification of migration behaviors and migration transitions

> Cancer aggressiveness assay: Quantification of contractility of somatic or cancer cells

> Endocytosis assay: Improved observation of events taking place at the membrane

> Exocytosis assay: Improved observation of events taking place at the apical membrane

> Frustrated phagocytosis: Characterization of the mechanism

> Immune system in a well: 2D migration and interaction of non-adherent immune cells

> Immune cells interaction: 2D interaction of non-adherent immune cells

> Mitotic assembly assay: Quantification of mitotic spindle disorders

> Quantitative cell migration assay: Fast and fine analysis of cell migration properties
 

文獻：

 

PUBLICATIONS

 

• Confinement and Low Adhesion Induce Fast Amoeboid Migration of Slow Mesenchymal Cells
  Y.-J. Liu, M. Piel, Cell, et al., 2015 160(4), 659-672
• Actin flows induce a universal coupling between cell speed and cell persistence
  P. Maiuri, R. Voituriez, et al., Cell, 2015 161(2), 374–386
• Geometric friction directs cell migration
  M. Le Berre, M. Piel, et al., Physical Review Letter 2013 111, 198101
• Mitotic rounding alters cell geometry to ensure efficient spindle assembly
  O. M. Lancaster, B. Baum, et al., Developmental Cell, 2013 25(3), 270-283
• Fine Control of Nuclear Confinement Identifies a Threshold Deformation leading to Lamina Rupture and Induction of Specific Genes
  M. Le Berre, J. Aubertin, M. Piel, Integrative Biology, 2012 4 (11), 1406-1414
• Exploring the Function of Cell Shape and Size during Mitosis
  C. Cadart, H. K. Matthews, et al., Developmental Cell, 2014 29(2), 159-169
• Methods for Two-Dimensional Cell Confinement
  M. Le Berre, M. Piel, et al., 2014, Micropatterning in Cell Biology Part C, Methods in cell biology, 121, 213-29