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產(chǎn)品型號(hào)44892

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AminoLink Reductant, 2 x 1 g 介紹

Thermo Scientific BCA蛋白定量產(chǎn)品可以用于研究蛋白：蛋白間相互作用，衡量親和層析后的組分含量，估算從細(xì)胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。

AminoLink Reductant, 2 x 1 g 現(xiàn)貨供應(yīng)

PIERCE	44892	AminoLink Reductant, 2 x 1 g.	2 X 1 G
PIERCE	44894	AminoLink Plus Immobilization Kit	1 KIT
PIERCE	44897	IgM Purification Kit	EA

AminoLink Reductant, 2 x 1 g 品牌介紹

美國(guó)Pierce公司(www.pierce.com)是蛋白質(zhì)化學(xué)、免疫學(xué)和層析等很多方面的和*。http://www.thermo.com.cn/Category828.html

主要產(chǎn)品包括：

1)化學(xué)發(fā)光體和比色底物

2)分析試劑

3)熒光標(biāo)記探針

4)酶聯(lián)免疫分析試劑盒等

AminoLink Reductant, 2 x 1 g 僅用于科研

Thermo Scientific AminoLink Coupling Resin is crosslinked 4% beaded agarose that has been activated with aldehyde groups to enable covalent immobilization of antibodies and other proteins through primary amines.

Proteins and other molecules with primary amines can be covalently attached (immobilized) to AminoLink Resin to make chromatography columns for use in affinity purification. The aldehyde groups form stable secondary amine bonds with primary amines such as exist in the side chain of lysine (K) residues, which are generally abundant and readily accessible in proteins. Once a protein is immobilized, the prepared affinity resin can be used for a variety of batch and column affinity purification methods involving binding interactions with the immobilized protein. The resin and linkage are stable in most binding and elution conditions typically used in affinity chromatography, enabling prepared resin to be used for multiple rounds of affinity purification procedures.

Highlights:

AminoLink Coupling Resin – aldehyde-activated crosslinked, 4% beaded agarose
Ideal for antibodies and other proteins – immobilize molecules via primary amines (-NH₂)
Flexible coupling conditions – efficient (>85%) coupling over a wide range of pH (4-10) and buffer conditions (PBS or other non-amine buffer with or without organic solvent)
Stable, permanent immobilization – Coupling reaction results in stable, leak-resistant secondary amine bond between resin and ligand
Better than immobilization to CNBr-activated agarose – bond is more stable and uncharged, resulting in less nonspecific binding in affinity purification procedures
Versatile and reusable – prepared affinity resin is adaptable to column and batch affinity techniques and the resin is reusable for typical applications based on protein binding interactions